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22-Jan-2018 20:43

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The cleavage reaction was stimulated by the presence of an RNA segment at the 5′-end of flap DNA.The 5′-end region of Okazaki fragments is efficiently processed by Dna2 when it is displaced from the template by the DNA polymerase (pol) δ extending the upstream primer (1).The possible mode of regulation of Dna2 is discussed based upon our recent finding that replication protein A interacts functionally and physically with Dna2 during Okazaki fragment processing.

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All recombinant proteins except HA-Dna2 contained an additional 37 amino acids (MPRGS HHHHH HGMAS MTGGQ QMGRDRW GS) at their N-termini.Further digestion generated two subtilisin-resistant core fragments of approximately equal size, 58 and 60 k Da.Surprisingly, digestion resulted in a significant (3- to 8-fold) increase in both ATPase and endonuclease activities compared to the intact enzyme.These included six histidines (bold) and the Xpress epitope (underlined) fused to its N-terminal methionine to facilitate detection and purification of the recombinant Dna2 proteins.



Evidence Suggesting that Pif1 Helicase Functions in DNA Replication with the Dna2 Helicase/Nuclease and DNA Polymerase δ. Martin E. Budd1,; Clara. To date, it has been impossible to determine which helicase, Rrm3 or Pif1, is the functional homolog of the single ortholog in other eukaryotes. One difference between.… continue reading »


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The latest Doric Nimrod Air Two Ltd DNA2 Ordinary Preference NPV share price DNA2. View recent trades and share price information for Doric Nimrod Air Two Ltd DNA2 Ordinary Preference NPV. Latest actual NAV date 30 September 2017. Premium/Discount 25.19%. 12m average Premium/Discount 25.11%.… continue reading »


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Apr 17, 2017. Here we demonstrate that DNA2 is overexpressed in pancreatic cancers, one of the deadliest and more aggressive forms of human cancers, where mutations in the KRAS are present in 90–95% of cases. In addition, depletion of DNA2 significantly reduces pancreatic cancer cell survival and xenograft.… continue reading »


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