Elucidating the mechanism of cellular
Due to the physical interactions between the labeled molecule and a protein, biochemical methods can be used to determine the toxicity, efficacy, and the mechanism of action of the drug.
Typically, computation inference methods are primarily used to predict protein targets for small molecule drugs based on computer based pattern recognition.
The A431 cell line expresses high levels of EGF receptors on the cell surface (2–3 × 10 receptors/cell) [23,24], and thus their adaptation with EGF could lead to changes in the cellular uptake ratio and pathway of PS NPs.
The harvested cells were treated with trypsin/EDTA for 12 min, centrifuged at 1200 rpm for 3 min, and then the cell debris was suspended in 1 m L PBS 1×.
Indeed, PS NPs have been reported to internalize in many types of cells, such as hepatocyte , macrophage , lung , and epithelium .
In contrast, there have been very few studies to assess the influence of cellular factors, such as growth factor responses, on the cellular uptake efficiency of nanoparticles.